FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18224376
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCC12600.014.62607475-79
GCTAGTC12750.014.11523775-79
GATCTAG13150.013.90416180-84
GGCTAGT13700.013.0313575-79
CTAGTTA14250.012.12314885-89
GAGCTAG14350.012.039391550-54
CTATTCT57700.011.9752325
TAACTAG15000.011.529404135-139
ACTATTC60500.011.4210044
TACTATT63400.011.2391753
ACTAGAT16750.011.185243135-139
AGGCTAG15750.010.42107775-79
CTAGATC16000.010.177054140-144
CTACTAT74700.09.8300082
TCTACTA79650.09.5539631
TCTAGTT19900.09.40457785-89
AGCTAGA19500.09.30274650-54
GCTAGAA21800.08.98168150-54
AACTAGA21100.08.606071135-139
TCTTGTG92250.08.5663011
Produced by FastQC (version 0.11.5)