FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17316210
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA350600.06.92013749
AGAGCAC373050.06.58088168
CTAGATC32000.06.345042140-144
CACACGT244800.05.9059215140-144
CACGTCT236800.05.5885506140-144
AAGAGCA448300.05.4922987
TTAGATC50950.05.4265327140-144
TCGGAAG498950.05.3161592140-144
AGCACAC313600.05.2898307140-144
CTCGTAT99950.05.27391840-44
GATCGGA551400.05.25183140-144
ACACGTC265700.05.2083144140-144
TAGATCG148200.05.1012483140-144
ACGTCTG251200.05.0446196140-144
Produced by FastQC (version 0.11.5)