FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16450885
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAA16900.010.6485613
ACCACTA62200.07.63954978
CCTAGAC14550.06.6311283120-124
GTTCCTA52550.06.16494132
TAGGCTA30652.4338078E-96.10705762
AGATCTA39702.7284841E-115.80294662
CCACTAC91350.05.59611279
TAGCTAA34052.656634E-85.49659633
CTAGACC19750.05.3956065120-124
CACCACT94900.05.234627
CTAGATC11700.05.0458064140-144
ATCCTAG23200.05.027194115-119
Produced by FastQC (version 0.11.5)