FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21887158
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAG18700.041.5861322
GACTAGC22250.034.9386563
CTAGCCT28800.029.4919155
ACTAGCC29350.027.4677034
CGGACTA73050.010.8440491
TAGCCTT134400.07.1765556
AGCCTTA148000.06.75848637
CTAGCTC32800.05.92712930-34
TACCACA117850.05.61868869
ACATACC127250.05.5434326
GCCTTAT179800.05.5231368
Produced by FastQC (version 0.11.5)