FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16401552
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGATTTCGTATCCCCAACCCAAATAAAAAAAGCCGCGCCGGCAAGGGCG420030.25609161864682073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCC151150.041.049219
TCGTATC150100.041.0006877
TTCGTAT160400.038.5922246
TTTCGTA168450.036.9188735
CGTATCC205200.030.482338
ATTTCGT211800.029.7023754
CCGATTT234400.026.9647881
CTAGGAG54900.022.294079105-109
GGCTAGG58250.021.415167100-104
GCTAGGA57200.021.296944105-109
AGGCTAG62550.019.942982100-104
TAGGCTA68400.018.132036100-104
GATTTCG381350.016.5988773
CGATTTC382950.016.5115782
GTAACAC80200.015.63627290-94
TAACACC82150.015.37027190-94
CAACCCA87300.014.51124615-19
TAGGAGC90650.013.867221105-109
AGTAACA93450.013.43465990-94
GACAGTA98050.013.00995585-89
Produced by FastQC (version 0.11.5)