FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15377672
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTC12450.015.14852475-79
GGCTAGT11900.014.75982675-79
AGGCTAG12300.013.81164175-79
CTAGTCC14300.013.08807375-79
GAGCTAG13700.012.61340750-54
AGCTAGA16350.011.27363550-54
TCTTGTG94050.010.4272241
GCTAGAA21500.09.37694550-54
CTTGTGG118050.08.5985032
AAAGGAC141400.07.48343379
CTAGAAA27850.07.445760350-54
TTGTGGA146350.07.2799813
TTAGAGC32600.06.35998245-49
TGTGGAA167700.05.7949064
CAAGTTA37300.05.790601360-64
GGAAAGG207750.05.50933277
AGTAGGG33751.0379881E-75.38096241
TGGAAAG203750.05.29946856
Produced by FastQC (version 0.11.5)