FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16121162
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGC12050.070.477073
GGACTAG11800.070.1417852
ACTAGCC12150.069.896584
CTAGCCT13750.062.2838755
TCTAGCT12750.015.24665725-29
CTAGCTC12700.014.6292130-34
CGGACTA61100.013.8309891
TTCTAGC16150.012.7501325-29
TAGCCTT77900.012.1961586
AGCCTTA78600.011.7223077
TTTCTAG18500.010.50783225-29
CCTTATT104600.09.22149859
GCCTTAT105150.09.104758
GCTCTAA30850.06.81604730-34
TAACTTG33300.06.14056915-19
CTTGCTA39750.05.615720320-24
CTCTAAA40300.05.110529430-34
Produced by FastQC (version 0.11.5)