FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14008501
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTTCAGTGTAGATCT421070.30058176817062726Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA214400.031.8513091
TCGGAAG243550.027.9137443
AGAGCGT261000.025.9307218
ATCGGAA274400.025.0380232
GAGCGTC274100.024.796499
AAGAGCG311800.021.8445077
CGGAAGA327800.020.937184
GGAAGAG377050.018.4079485
GAAGAGC381600.018.1507246
CAGTGTA102200.013.45578735-39
TAGATCT110300.012.83305240-44
AGTGTAG111650.012.41994140-44
TCAGTGT113400.012.38078435-39
TTCAGTG124650.011.30959535-39
GTAGATC129800.011.08262940-44
GAGTGTT130900.010.57233825-29
AGATCTC134900.010.52491245-49
AGTGTTC131950.010.43363925-29
GTAGGGA143100.09.81153115-19
TGTAGGG143750.09.78720215-19
Produced by FastQC (version 0.11.5)