FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16635629
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT52700.015.98105058
CACTATA54450.014.8064269
GCTAGTC12300.011.70460675-79
AACACTA45400.011.4160837
GAGCTAG13550.09.98988250-54
GGCTAGT15550.08.88797475-79
CTAGTCC17950.08.10063175-79
GAGCACA176600.07.25536739
CACACTA21051.6008926E-87.1813777
TCTTGTG102150.07.11281441
AAACACT80950.06.75827656
CACCTAG10250.06.7423840-44
AGAGCAC182950.06.72812038
AGGCTAG19800.06.689359775-79
GATCGGA194050.06.4775731
AAAGGAC138500.06.08087649
TCGGAAG221800.05.93947943
CCAAACA91500.05.82184034
TGTGGAA147600.05.65745744
ACCAAAC94900.05.5375223
Produced by FastQC (version 0.11.5)