FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14007022
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT43900.021.5199721
GTGAGTA49000.018.666272
TAACCCC52800.017.4541539
GTCTCTT71300.012.2385721
ATAACCC88750.010.7896178
GAGTATA108550.09.2220424
TGAGTAT107700.09.0274123
GTATAAC113100.08.7848856
TATAACC133950.07.47127637
CCTAGGG9450.06.55230865-69
CTAACTA32550.06.370186125-129
AGAGCGT178000.06.06727938
ACCTAGG10500.06.03421965-69
GATCGGA183100.06.0261231
CTAGGGG9900.05.9635765-69
GACCCTA33700.05.939285885-89
CTCTTCA152800.05.8915813
CGCCTAA36150.05.815612345-49
TCTTCAG165150.05.71261364
CTTCAGC176300.05.47232775
Produced by FastQC (version 0.11.5)