FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15359159
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCC46450.014.2600769
GATCTAG6453.962301E-612.2906313
AGTGAAT70050.010.2880511
GGCTAGG11600.09.31033100-104
GTCTCTT76200.08.9848291
GCTAGGA13250.08.368573105-109
GTGAATA82950.08.25369552
AGGCTAG12850.08.180532100-104
GTCCCTA20801.551598E-97.62255533
TATAAAC94150.07.49422747
TCTAGGA19800.07.345247750-54
GTATCCC92250.07.18027649
ATAAACC94350.07.09677038
TTCTAGG20450.06.90053950-54
CTAGGAG20100.06.80577850-54
TCGTATC96400.06.4230687
CTAACTA24000.06.119851125-129
ATCTAGC12950.00751901056.11566974
GTTCTAG25450.05.88431350-54
TTCGTAT103250.05.78774176
Produced by FastQC (version 0.11.5)