FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15082289
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA43400.035.9990359
GCCACTC76500.021.4582488
CCCTTAA66250.018.1487064
AAGCCAC96300.016.821986
AGCCACT102150.015.7881247
ACACTAT52100.015.3393218
TCCCTTA82300.015.1344173
AACACTA52300.014.5923437
TACAAAG132700.012.8586932
GTTCCCT100150.012.5806661
CCTTAAA106100.011.8753115
CTTAAAT102150.011.770616
ACAAAGC155100.011.0480233
CTCACTA30250.010.71057715-19
ATACAAA166800.010.4888241
CACTATA82800.010.0866869
AAACACT77800.09.9945916
AAAGCCA180800.09.5971195
GGCCTAA36000.09.36046375-79
ACCAAAC88250.09.0558183
Produced by FastQC (version 0.11.5)