FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005091993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005091993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13944775
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA43350.019.9276497
ACACTAT48800.017.5546058
CACTATA51150.016.0440439
AAACACT71000.012.1670496
ACCAAAC83250.011.0707463
CCAAACA92200.09.60561854
CAAACAC94050.09.0320355
TACCAAA128400.07.40664962
TATTTAG56250.06.910769
GCCCTTA58450.06.5289374
TAGCCCT65050.06.20231252
ATACCAA155700.06.18401531
TTAGGCA36950.05.299222560-64
CTATAGC38050.05.146095810-14
Produced by FastQC (version 0.11.5)