FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14270962
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAG32050.015.0511715
CCCCTTA38200.012.6276584
TAGAGCC48550.09.6392479
GCACATA90350.09.4030073
CACATAA103850.08.6659244
TTAGAGC60450.08.21816358
TGCACAT104050.08.1649362
CCTTAGA64400.07.60236
ATGCACA127650.07.10656171
CTTAGAG72350.06.76694157
ATCACAC58900.05.86743078
TGCCCCT80700.05.79899452
GCCCCTT88350.05.62283563
TCACACC60550.05.23187649
CTAACCC34450.05.225137755-59
Produced by FastQC (version 0.11.5)