FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19127893
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG333020.17410176855338955No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA225750.11802136283384687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG27000.013.52837851
CTAACGG42850.09.0820172
TACCAAG110900.07.44365841
CCTGTAG79650.07.41103847
GATCCAT96250.06.2130423
CTCGAAT92950.06.04605533
ACCAAGA131900.06.01017762
GTGATTC114400.05.8548184
CTGTAGT103500.05.77281438
AGATCCA103750.05.69593672
TAGTCCC55450.05.192889
CGTGATT122750.05.16521173
TGAGCGG164500.05.10706571
TGTAGTC121600.05.0319379
Produced by FastQC (version 0.11.5)