FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14265735
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTCC22000.09.490245
CTAGGGG4850.08.90652635-39
TAGTCCC30900.07.68867066
TGATTAG30550.07.54223442
CCCTAGG5950.07.259941635-39
CCTAGGG6000.07.199442435-39
GTAGACA58000.06.87309361
GATTAGT35650.06.66521453
ATTAGTC31851.382432E-106.32940154
TCCCTAG9050.05.886836535-39
AGTCCCC47802.7284841E-115.271417
Produced by FastQC (version 0.11.5)