FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12616717
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAAAGCCACTCAACTCACTATATAGCCACTATACCATATAGCTCTAC161280.12783040152204414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA64100.036.0552989
GCCACTC72800.032.1420868
AAGCCAC106400.021.9921286
AGCCACT116950.020.0697947
ACAAAGC121900.019.2721563
TACAAAG178550.013.6418492
GGCCTAA38400.012.78719375-79
ATACAAA195350.012.4686571
CCGTTAA48350.012.3596774
AAAGCCA208450.011.8127155
CGTTAAA51850.011.5253695
CTCACTA42450.011.33016715-19
CAAAGCC229450.010.8884734
TACACAC48600.010.221992550-54
CATTAGG50500.09.837512100-104
GGGCCCC52050.09.54447110-114
CTACACA52300.09.388750-54
GGGGCCC53900.09.217139105-109
ACACACT56350.08.94389950-54
ACTCACT57400.08.68024115-19
Produced by FastQC (version 0.11.5)