FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14499584
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGG21450.015.977427570-74
CCTAGGG22700.015.73206365-69
ACCTAGG23100.015.58432265-69
GCTAGTC12951.8189894E-1211.6750018
TGTCCAG162000.011.208871
ACGTCGA110400.010.630923
TCACGTC110000.010.52277851
AGCGCAA174950.010.4115156
GCGCAAA185500.09.9746267
CACCTAG39050.09.73515265-69
GGGCTAG18251.0913936E-119.0734486
CTAGTCT18501.4551915E-118.9508969
CGCAAAA213450.08.8371568
GGACTAA32400.08.79952810-14
GGTGTCA93150.08.4653861
GCAAAAC226400.08.3635329
TCGAACG147350.08.3551336
CACGTCG139150.08.3309882
GGTGTAG117300.08.3260481
GGTGTAA77950.08.2599421
Produced by FastQC (version 0.11.5)