FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15473525
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAC100000.011.3827187
CTAGAGT12650.010.468202140-144
GTGCCAG130150.09.9636227
GACTACT70000.09.8801178
AGGACTA58700.08.9592916
CTCAGAG37800.08.4366961
TGGACTA66800.07.5493666
CTAGCTG14103.2506476E-57.36384531
TAGCTTG48100.07.092651
ACTAGGG9900.06.987166410-14
GCTAGAG16800.06.8541803140-144
CTACTAG10150.06.53110810-14
TGCCAGC229050.06.16473878
CTAGGGT11600.05.96318510-14
TCTAATC42850.05.949390420-24
ACTACTA63000.05.48916679
GGTGCCA110300.05.4864666
TTGGACT66000.05.45446635
AGCTAGA15500.05.200333140-144
TCAGAGG61050.05.06958962
Produced by FastQC (version 0.11.5)