FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17179728
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA42400.023.6022197
CACTATA45750.022.5030429
ACACTAT45650.022.0795978
GTCCTAA28550.018.92122
AGTCCTA41950.013.8772771
AAACACT83300.012.5324076
TAACAAA102050.010.5869722
CTAAAAG58300.010.0028595
CAAACAC109200.09.36210255
TCCTAAA68300.09.0653683
ATAACAA118200.09.0499481
AACAAAC139200.08.43055253
ACAAACA134800.07.8511834
ACCACTA52100.07.32389358
CCTAAAA81900.07.2962864
TACGTTA35900.06.8185523
TATTTAG54700.06.58080439
AAAGTTA97600.06.34384448
ACTATAG35900.06.01614910-14
CACCACT73850.05.94679837
Produced by FastQC (version 0.11.5)