FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16647424
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGATTACTCGCGTGTAGATCTCGGT369690.22207039359362749Illumina Single End PCR Primer 1 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA217250.027.5275081
TCGGAAG239100.025.0206183
ATCGGAA261850.022.9358772
AGAGCGT259950.022.73848
AAGAGCG289900.020.611297
GAGCGTC312050.019.1956659
GAAGAGC347700.017.4748866
GGAAGAG349350.017.2687075
CGGAAGA372450.016.1203584
TTACTCG90050.012.9030325-29
TAGATCT99800.011.9456540-44
ACTCGCG113650.010.55304930-34
ATTACTC116550.010.32751625-29
CTCGCGT118050.010.31826830-34
TCGCGTG123950.09.96651130-34
CGCGTGT128950.09.73638130-34
TACTCGC125000.09.72153530-34
GTAGGGA127050.09.632615-19
TAGGGAA135500.09.20190715-19
GTGTAGA138250.09.1857835-39
Produced by FastQC (version 0.11.5)