FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18621118
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTTG55550.018.2058641
TGGACTA78800.013.8854076
GGTGCCA86850.013.59299856
CTTGGAC75100.013.5155724
TTGGACT77900.012.9373665
GTGCCAG116650.012.2796577
GGACTAC124500.010.5225257
GCTTGGA107600.09.9013993
AACACTA50300.09.4448247
AGGTGCC136050.09.1009175
CACTATA53850.08.823829
TCAGGTG136900.08.4658293
ACACTAT59400.08.3621268
GACTACT72950.07.99307358
AGCTTGG143850.07.66116242
CTAGGGT11000.07.592245610-14
TGCCAGC220550.07.0501898
ACTAGGG12700.06.235824610-14
AAACACT92500.05.99225436
ATCAGGT215100.05.79320342
Produced by FastQC (version 0.11.5)