FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18510649
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA196300.014.9980411
AGAGCGT211650.013.9042718
GAGCGTC219800.013.7487999
AAGAGCG243400.012.4452817
TCGGAAG280850.011.1956123
GAAGAGC314350.010.1398126
ATCGGAA324750.09.7946262
TAGATCT67700.09.14212840-44
GGAAGAG377900.08.8915955
CGGAAGA459000.07.1481584
GTAGATC98550.06.645417740-44
GAGTGTC97650.06.425525-29
GTAGGGA104600.06.13593215-19
AGAGTGT108600.05.94989325-29
AGATCTC108350.05.925357345-49
TGTAGGG112950.05.66958415-19
AGTGTCA116750.05.48524225-29
TAGGGAA121150.05.32147515-19
GTGTAGA123900.05.26252840-44
Produced by FastQC (version 0.11.5)