FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16210614
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA164200.012.7586029
GTATAGA91300.011.8203351
AGAGCAC183200.011.5925728
GTATAGG75600.011.2850361
GTATACA55450.010.7832871
GTATAGT71400.010.4169561
GTATATC138950.010.1808041
GTATATT151400.010.1142191
GTATTAA58850.09.9124821
GATCGGA228050.09.8802361
GTATACG68000.09.5437711
GTATATG128200.09.4418911
GTATAGC78900.09.4267511
GTATAAC69050.09.0818361
GTATGAA127350.08.9895841
GTATACC56150.08.9606231
TCGGAAG244900.08.9424143
GTATGGA137600.08.8498711
GTATATA94100.08.446481
GTATTCT147700.08.3434391
Produced by FastQC (version 0.11.5)