FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16210614
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATC139450.010.4739581
GTATACC59200.09.8688741
GTATTAA57100.09.8481341
GTATAGA93100.09.72683051
GATCGGA233700.09.5310431
GTATAGG73400.09.4520691
GTATATT139150.09.2369551
GTATTGA161650.09.1710621
GTATATG124100.08.8859631
GGTACCT44650.08.8322551
GTGTAAG41750.08.7460681
GTATAGC73850.08.7022491
TCGGAAG252750.08.6035823
GTATAAA109700.08.587811
GTATACG68400.08.5414821
GTATATA94250.08.5233561
GTATAGT67800.08.4016431
GTATTGT120250.08.3809521
GTATCGA163400.08.357741
GTATGAA124850.08.3061381
Produced by FastQC (version 0.11.5)