FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29029012
Sequences flagged as poor quality0
Sequence length98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3215051.107529942803427No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG788930.2717729421862514No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT505020.17397078481348247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT524400.067.229741
GTATCAA579900.060.7030569
AGCAGTG598500.058.920892
GGTATCA598600.058.7065968
TGGTATC602850.058.2777637
GTGGTAT618950.056.8434226
CAGTGGT692100.050.9623764
AGTGGTA717000.049.309155
GCAGTGG787150.044.936913
ATCAACG562850.033.8408410-11
CAACGCA575100.033.12669812-13
ACGCAGA610450.031.29842614-15
TCAACGC571400.030.87744912-13
AGAGTAC616200.030.8768118-19
AACGCAG617250.028.65054914-15
GCAGAGT676950.028.31069416-17
TATCAAC642250.027.58331710-11
AGTACTT693200.027.3434320-21
GTACTTT639000.027.26617822-23
CGCAGAG661850.026.7879816-17
Produced by FastQC (version 0.11.5)