FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29326998
Sequences flagged as poor quality0
Sequence length98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2937401.0016026870530697No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG631880.21546017086372088No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT456430.15563474993246837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT496800.065.1699451
GTATCAA538150.060.17459
GGTATCA557350.058.0845578
TGGTATC563700.057.4386027
AGCAGTG567300.057.1776772
GTGGTAT576650.056.229146
CAGTGGT651100.049.8689164
AGTGGTA677850.047.996535
GCAGTGG751400.043.3221133
ATCAACG521050.033.38807710-11
CAACGCA535600.032.48491312-13
ACGCAGA569700.030.55169714-15
TCAACGC530100.030.50480812-13
AGAGTAC573800.030.33419418-19
AACGCAG572000.028.3498614-15
GCAGAGT633550.027.56929616-17
TATCAAC604250.027.05111910-11
GTACTTT600600.026.90159822-23
AGTACTT654150.026.5789520-21
CGCAGAG619300.026.19813316-17
Produced by FastQC (version 0.11.5)