FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28739787
Sequences flagged as poor quality0
Sequence length98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3075271.0700392455935739No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1051330.3658099484175022No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT467670.16272563189142633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT507900.066.7954561
GTATCAA557100.061.0160189
GGTATCA576050.059.0558138
TGGTATC577300.058.808077
AGCAGTG577650.058.71792
GTGGTAT594900.057.1385656
CAGTGGT665700.051.011384
AGTGGTA696600.048.9941145
GCAGTGG763350.044.688543
ATCAACG541500.033.80051410-11
CAACGCA553400.033.0689812-13
ACGCAGA588300.031.18467314-15
TCAACGC548750.030.97190912-13
AGAGTAC592950.030.95216218-19
AACGCAG595650.028.64071314-15
GCAGAGT648600.028.32624216-17
TATCAAC619300.027.6484110-11
GTACTTT620300.027.3317322-23
AGTACTT670150.027.28496220-21
CGCAGAG633000.026.94181416-17
Produced by FastQC (version 0.11.5)