FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005092793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52951081
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACACT1831806334.59431357029331No Hit
GTACGCGG1393951726.325273699322587No Hit
CCTTTGTC1355266125.594682382404997No Hit
TAGGATAA44109248.330186875693812No Hit
AGCACCCT2517070.47535762301056705No Hit
AGCCCACT1874140.3539380055338247No Hit
GTCCGCGG1810310.3418834829830953No Hit
CCTTTGTA1580250.29843583363293374No Hit
CGCACACT1331700.251496282011693No Hit
GTAAGCGG1238120.23382336613675556No Hit
AGCACAAT931890.17599074134105025No Hit
GTACGAGG828120.15639340771909832No Hit
AGAACACT816850.15426502813039833No Hit
AGCAAACT701820.13254120345531756No Hit
AGCACACA699010.13201052495982094No Hit
AGCACACG628450.11868501796969924Illumina Multiplexing Adapter 1 (100% over 8bp)
ACTTTGTC617610.11663784541055923No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)