FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62100212
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGG1324810.2133342153485724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT152300.017.2892191
ATGCGAT179700.017.2408141
CCTTACG215300.017.046941
TGAGCCG185100.017.0403961
GACCTGG197900.017.0044921
CGGACTG156800.016.8313031
CGCTGGA222150.016.79141
GAAATGA257900.016.7593771
CAATGGA232900.016.6936978
GGGAGAT210550.016.6335641
GAATGAA182300.016.632861
CCCTCCT290400.016.629721
TGGCTGG214400.016.605411
GGCGACT137100.016.5727771
CCACTAC279350.016.567961
GGATTAC224350.016.5554221
TGCTACC226100.016.5157451
TACGGAT189850.016.503431
CGGAGCT156100.016.4967651
TGACGGC162450.016.4552171
Produced by FastQC (version 0.11.5)