FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31994353
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCA1136340.35516892621644824No Hit
CGTTCGTTATCGGAATTAACCAGACA508860.15904681679295093No Hit
CCACCTACTAAGAACGGCCATGCACC425540.1330047211768902No Hit
CATGCCTGTGTTGGGTTGACAGTGAG407040.1272224507868623No Hit
CCTTTAAGTTTCAGCTTTGCAACCAT331700.10367454531741897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCAG745900.019.6309091
GGCGACT359450.019.518071
TGGCTGG524900.019.4524231
GACTGCG383800.019.4473271
TGAACGA709850.019.3750442
TCGGTAA521450.019.3624461
GAACGAG709950.019.3554443
TATGCCC393550.019.3238961
CCGGGAT373900.019.314851
GGCCAGC549750.019.283842
GACTAAC250150.019.2613071
GGAATGC595450.019.20343810
CGCTGGA445550.019.1833841
CTCAGAC505900.019.18236
AACGAGG707900.019.1685454
CTGGATC430950.019.1675113
GATCGAG436750.019.1672156
TGGATCG428800.019.1656974
CATGCGC386700.019.165252
GCATGCG405150.019.1607041
Produced by FastQC (version 0.11.5)