FastQCFastQC Report
Thu 12 May 2022
EGAF00005092828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37801213
Sequences flagged as poor quality0
Sequence length98
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1238050.327515945057107Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG997760.2639492018417504No Hit
GGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTA720080.19049124164348907No Hit
GGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATA710670.18800190353679921No Hit
GCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAG707470.18715536985545939No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGT687950.1819915143992866No Hit
GTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAA657200.17385685480516194No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC654490.1731399465937773No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTA652190.17253150051031432No Hit
CTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA631980.16718511122910262No Hit
GTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAG631100.16695231446673417No Hit
GGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT597370.15802932038186182No Hit
GGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT557630.14751643022672314No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTC557510.1474846852136729No Hit
GGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTA522980.13835005770846562No Hit
AGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATG515380.13633954021528358No Hit
GTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAGTTAG499680.13218623434121016No Hit
CTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTA491480.13001699178277693No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT476320.1260065384674296No Hit
GGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCC475880.12589014008624538No Hit
CTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGG463580.12263627624859552No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTT448990.11877661174523686No Hit
GCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT443320.1172766598786129No Hit
GTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGC441760.11686397470895973No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT436390.11544338537496138No Hit
GTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATG430210.1138085172028739No Hit
GTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGT430040.1137635451010527No Hit
CTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGG406420.1075150683656633No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT404820.10709180152499338No Hit
CCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTT402660.10652039129008903No Hit
AATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTG401470.10620558657734079No Hit
GGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTA386630.10227978663012743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)