FastQCFastQC Report
Thu 12 May 2022
EGAF00005092848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36801590
Sequences flagged as poor quality0
Sequence length98
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG893560.24280472664360425No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT866440.23543547982573576Clontech SMART CDS Primer II A (100% over 26bp)
GCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAG696630.18929345172314566No Hit
GGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTA690210.18754896187909273No Hit
GGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATA685190.18618489038109495No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC645660.17544350665283756No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGT643000.17472071179533277No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTA628690.17083229284386897No Hit
GTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAA628540.17079153373536307No Hit
CTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA613100.16659606283315476No Hit
GTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAG611610.16619118902199606No Hit
GGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT573260.15577044361398515No Hit
GGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT538640.1463632413708212No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTC528380.143575318349017No Hit
GGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTA506820.13771687581976758No Hit
AGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATG505220.13728211199570453No Hit
GTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAGTTAG484500.13165192047408822No Hit
CTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTA478340.1299780797514455No Hit
GGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCC468700.12735862771146572No Hit
CTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGG449120.12203820541449432No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT446550.12133986602209307No Hit
GCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT434660.11810902735452462No Hit
GTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGC428080.1163210611280654No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTT426490.11588901457790274No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT420800.11434288572857856No Hit
GTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGT418910.1138293209614041No Hit
GTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATG417340.11340270895904227No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT397070.10789479476294367No Hit
AATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTG395410.10744372729547826No Hit
CTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGG394510.1071991726444428No Hit
CCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTT390150.10601444122387102No Hit
GGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTA378150.10275371254339827No Hit
ATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTAT368950.1002538205550358No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)