FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38141144
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACA393590.10319302430991581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTACG209200.017.8160151
CCGTGGA180850.017.8101181
ATGCGAT125250.017.5701071
GACTAAC125300.017.5630931
CAATGGA196900.017.4042788
TGACAAC150650.017.3699281
TGCGGGT132950.017.2672731
ACTGATG110900.017.2458951
GGGCATC155350.017.0955351
CCCTCCT200850.017.0576081
CGTTGGG183350.017.0326181
CGGACAC137500.017.015981
TGACTAG124550.016.9380051
TCCCGAT124850.016.801161
GTCGTAA164250.016.796481
CGGACTG91550.016.771791
AAACTAG119050.016.6817653
CGCTTCA139350.016.6608661
TATATGA91600.016.6480649
TGTAAAC122250.016.6457427
Produced by FastQC (version 0.11.5)