FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58674641
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGG2123730.3619502333214105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT169150.017.067521
CGGACTG146500.016.9827631
GCGCGAT141100.016.9665181
TGAGCCG177050.016.9216041
CCCTCCT278750.016.9002781
TGCTACC213550.016.8670751
GAAATGA241350.016.842591
CCTTACG207250.016.7042961
GGCGACT128950.016.6729621
GACCTGG187450.016.6709441
CAATGGA220800.016.6219888
ACGAGCC172600.016.558351
GAATGAA173300.016.5087781
CCGGGAT118750.016.4882561
GGATTAC207150.016.4854721
GGGAGAT196050.016.4752241
CGCTGGA205050.016.449481
TACTTGT192150.016.4349121
ATCGAGT144650.016.3980371
CCACTAC261350.016.3801691
Produced by FastQC (version 0.11.5)