FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29129710
Sequences flagged as poor quality0
Sequence length98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2159550.7413565050939401No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG953070.32718142405125217No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT360920.1239009931784422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT397800.058.7751921
GTATCAA414250.056.443059
GGTATCA436350.053.668718
TGGTATC438000.053.3402987
GTGGTAT451200.051.912556
AGCAGTG504650.046.4789432
AGTGGTA547500.043.0554665
CAGTGGT597950.039.3302084
GCAGTGG718650.033.006053
ATCAACG388250.032.49205410-11
CAACGCA406100.031.09719712-13
TCAACGC396050.029.51080512-13
ACGCAGA434750.029.2220714-15
AGAGTAC441650.028.58540218-19
AACGCAG439300.026.72544914-15
TATCAAC448600.026.14157510-11
GCAGAGT517200.024.67805316-17
GTACTTT473450.024.56666822-23
AGTACTT522900.024.20882820-21
CGCAGAG488800.024.05549616-17
Produced by FastQC (version 0.11.5)