FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29507320
Sequences flagged as poor quality0
Sequence length98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1979260.670769151519013No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG779800.2642734074121269No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT325570.11033533374091582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT384450.057.4831121
GTATCAA394850.055.622569
GGTATCA413800.053.0536278
TGGTATC420350.052.237617
GTGGTAT432300.051.0070576
AGCAGTG479850.046.075822
AGTGGTA523850.042.320995
CAGTGGT565250.039.09094
GCAGTGG683350.032.6914333
ATCAACG374500.032.2585410-11
CAACGCA390150.031.00559412-13
ACGCAGA420900.028.90931914-15
TCAACGC383550.028.70846412-13
AGAGTAC430550.028.26896518-19
AACGCAG423600.026.12405214-15
TATCAAC434250.025.60581610-11
GTACTTT458150.024.08112522-23
AGTACTT507200.023.90002420-21
GCAGAGT514150.023.7768116-17
CGCAGAG472500.023.5312516-17
Produced by FastQC (version 0.11.5)