FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28813726
Sequences flagged as poor quality0
Sequence length98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3277871.1376071251597242No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG774240.2687052691484607No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT514640.17860931973879393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT520100.067.600961
GTATCAA579950.060.4600339
AGCAGTG590600.059.530212
GGTATCA596500.058.75888
TGGTATC600700.058.3629847
GTGGTAT618900.056.7278066
CAGTGGT694850.050.6828464
AGTGGTA721650.049.0310135
GCAGTGG783300.045.1968963
ATCAACG560700.033.80316510-11
CAACGCA574400.033.067812-13
ACGCAGA612500.031.0969814-15
AGAGTAC612100.031.04153618-19
TCAACGC566600.030.98899812-13
AACGCAG616650.028.58921614-15
GCAGAGT672900.028.39222516-17
TATCAAC642200.027.46022210-11
GTACTTT639750.027.2185422-23
AGTACTT697200.027.17231220-21
CGCAGAG657900.026.82942616-17
Produced by FastQC (version 0.11.5)