FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40653542
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGTTATCGGAATTAACCAGACA467580.11501580846264269No Hit
GTCTCGTTCGTTATCGGAATTAACCA432260.10632775859973037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTCA199250.017.6832261
CTCGAAA131100.017.4999941
CCGGGAT125100.017.4679241
GACTAAC105050.017.3079241
GGGACCT116150.017.2812561
CCTTACG159100.017.2426011
ACTGAAC104500.017.1884691
AAACCTG156050.017.1822571
TGAGCCG127900.017.1637081
CCTACCA188400.016.992291
GACCAAT135150.016.9903771
GTGCAGC154150.016.9204221
CTGCCTA151700.016.890431
GGGTTGC164700.016.8810081
TGCGGGT117750.016.8595811
CGGACTG97550.016.8342231
CCACCTA177250.016.7860261
CGATTGA111950.016.7682341
GGGAATG113600.016.7359731
ACTTGAA103700.016.70190810
Produced by FastQC (version 0.11.5)