FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005092937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28921663
Sequences flagged as poor quality0
Sequence length98
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2199950.7606581958997309No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG942630.32592524157411No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT368340.12735782171308752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT398100.059.3347851
GTATCAA416000.056.619329
GGTATCA437400.053.985418
TGGTATC440400.053.7637757
GTGGTAT449200.052.7408946
AGCAGTG496800.047.677242
AGTGGTA542900.043.976445
CAGTGGT586850.040.6434334
GCAGTGG712650.033.7563133
ATCAACG394400.032.53181510-11
CAACGCA411450.031.15496612-13
TCAACGC401900.029.39334712-13
ACGCAGA444550.028.9797514-15
AGAGTAC449800.028.5709218-19
AACGCAG444150.026.63316214-15
TATCAAC455200.026.02308310-11
GCAGAGT525750.024.6552216-17
GTACTTT476900.024.5836322-23
AGTACTT528900.024.23634720-21
CGCAGAG495500.023.96876516-17
Produced by FastQC (version 0.11.5)