FastQCFastQC Report
Wed 11 May 2022
EGAF00005092949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005092949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32329686
Sequences flagged as poor quality0
Sequence length98
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1658020.5128475420392268Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1153130.35667837912190054No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT512390.15848901223476158Clontech SMART CDS Primer II A (100% over 24bp)
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC404560.12513576531488738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)