FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16738575
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTAACTCGTGTAGATCT219000.13083551019127973Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA221600.018.4166071
TCGGAAG237450.017.0046753
AGAGCGT246950.015.7377128
ATCGGAA273600.015.0736162
AAGAGCG297050.014.0040147
TAACTCG60050.013.49619535-39
GATCTAG11850.013.4869580-84
GCTAACT58750.013.35553830-34
CTAACTC60650.012.88966930-34
GAGCGTC299250.012.8433489
CTAGTTA12450.012.83724585-89
TCTACTA59350.012.729381
ACTATTC66200.012.5030574
CTATTCT68650.011.9518955
TGCTAAC68500.011.87491930-34
GAAGAGC361100.011.660066
GTGCTAA72200.011.42589530-34
GGAAGAG366600.011.3869655
GCTAGTC9900.010.61724575-79
CGGAAGA387300.010.5926284
Produced by FastQC (version 0.11.5)