FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16826215
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTC15100.010.58401375-79
GGCTAGT17950.08.66290775-79
GAGCTAG16600.08.50003650-54
CTAGTCC19400.07.79277175-79
AGGCTAG20650.07.669674475-79
TCTTGTG100350.07.21158461
CTTGTGG124400.06.5422022
CTAGATC24850.06.490417140-144
CACTATA57100.05.7990989
TAACTAG28950.05.770393135-139
ACACTAT49000.05.72937448
ACTATTC85050.05.50134234
AAAGGAC155900.05.26377639
CTATTCT88400.05.21143535
GATCTAG14550.05.145882680-84
TTGTGGA168000.05.14443733
AACACTA53605.456968E-125.10337547
Produced by FastQC (version 0.11.5)