FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12828024
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT28050.027.4729251
GTGAGTA35750.022.1612112
TAACCCC44750.017.3734389
TAGCATA77450.012.269058
ATAACCC70250.011.5795198
CCTTTAG85050.011.2567684
GTATAAC104000.08.5139326
TGAGTAT99650.08.379483
GAGTATA104700.08.2503244
TTAGCAT118950.08.2306267
CTTTAGC132550.07.4400745
TATAACC117100.07.25409947
GTTCCTT154550.07.24408671
AGCATAT158400.06.4533949
TTTAGCA163400.06.34408336
TCCTTTA178400.06.05247263
CGCCTAA32350.05.65346945-49
CTAACTA32800.05.619202125-129
AACCCCC36200.05.050883310-14
AACCCTA39250.05.0253185-89
Produced by FastQC (version 0.11.5)