FastQCFastQC Report
Fri 27 May 2022
EGAF00005093075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343204735
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGGGGGG35716721.0406826117943857Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGGGGGGG32296040.9410138237166221Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGGAGGG15068710.43905891916089096Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAGGGGG14814220.43164381167410176Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGGGG13860300.4038493233492248Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAGAGGG11989070.3493270569242001Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGAGGGGG11591190.337733976776282Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGAGGGG10725570.31251229677818987Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGAGGGGGG9726350.28339789659370523Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGGGAGGG8393770.2445703437046112Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAAA8120170.23659842571810671Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGGGGGA7268350.21177883807459705Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGGGGAG7224370.21049738722281908Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAGAGAG6502510.18946446062289904Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAGAGGA6396390.1863724286904142Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGAGAGGG5979870.1742362324925383Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGGAGGGG5751920.16759442436014177Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAGGGG5398800.15730552202317372Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAGAA5207630.15173537742712087Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAGGGAG5023500.14637035820615935Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGGAGGA4976730.14500761476965054Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGGAGAG4946860.14413728878186952Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAGGGGA4924900.14349743746979482Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGAAGGG4833290.14082818525216445Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAGGG4623710.13472162614539684Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAGGA4453250.12975491145248913Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAGAG4206340.12256066338944888Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGGGGGGA4022010.11718981674305863Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGAGGGA3960820.11540691593313827Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAGAGAA3848920.11214647140576309Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGGCGGG3736210.10886242580540155Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAGGGGGAG3490690.10170867834909098Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAGAGGAG3440370.10024249811122214Illumina Single End PCR Primer 1 (100% over 56bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)