FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005093078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343802426
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6869492519.980930850092374TruSeq Adapter, Index 6 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA39621821.1524589998093848TruSeq Adapter, Index 6 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC35550371.034034879090702TruSeq Adapter, Index 6 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16028040.4661991535801437TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15970710.464531626079916TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15645640.4550764862840148TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15336020.4460707324968091TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15240250.4432851209723575TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14604560.42479514091619586TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14429790.4197116980204206TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14207560.4132478111134678TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14081940.4095939683683326TruSeq Adapter, Index 6 (98% over 62bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA13661110.3973535079127103TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA13402300.3898256378214155TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA11987430.3486720596904688TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA10911470.31737617814250096TruSeq Adapter, Index 6 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4094360.11909049181636665TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3963560.1152859811408079TruSeq Adapter, Index 6 (98% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)