FastQCFastQC Report
Tue 5 Jul 2022
EGAF00005093084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336049571
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA7018790520.886176045735823TruSeq Adapter, Index 6 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA40177321.1955771846529155TruSeq Adapter, Index 6 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC33588780.9995186097113036TruSeq Adapter, Index 6 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16356050.48671539592591834TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16323430.485744705801157TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16003170.47621456418999564TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15648880.4656717743585514TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15556160.46291265760907635TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14939040.44454870022732446TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14744380.4387561024441837TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14505300.4316416758645409TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14406870.42871264370696077TruSeq Adapter, Index 6 (98% over 62bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA13872830.4128209406343804TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA13674630.4069230012497174TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA12204580.3631779669791633TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA10751710.3199441668086522TruSeq Adapter, Index 6 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4603380.13698514734899037TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4175140.12424178931625537TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAT3371070.10031466458857642TruSeq Adapter, Index 6 (100% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)