FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005093086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348719191
Sequences flagged as poor quality0
Sequence length75
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA7251150020.793664894685993TruSeq Adapter, Index 6 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA41266161.1833636078835708TruSeq Adapter, Index 6 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC36633651.050520044364292TruSeq Adapter, Index 6 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16920010.4852044406125041TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16859580.4834715276682321TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16502530.4732326303200216TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16137220.4627568661685729TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16106520.46187650165774785TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15418990.4421606380705328TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15222180.4365168419996708TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14991750.42990894642216576TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14883770.42681247215901TruSeq Adapter, Index 6 (98% over 62bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14278470.4094546663478581TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14123940.4050233071342495TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA12570750.36048345845124424TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA10605600.30413009302949434TruSeq Adapter, Index 6 (100% over 62bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6130920.1758125207396458TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4676670.13410991194918206TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4387370.12581383856215703TruSeq Adapter, Index 6 (98% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)