FastQCFastQC Report
Fri 27 May 2022
EGAF00005093092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359906915
Sequences flagged as poor quality0
Sequence length75
%GC22

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA144818684.023781538067976TruSeq Adapter, Index 6 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8925880.24800523768763932TruSeq Adapter, Index 6 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC8861340.24621199623241471TruSeq Adapter, Index 6 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6812080.18927338475838953TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA6516260.181054037264052TruSeq Adapter, Index 6 (98% over 62bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4045490.11240378640682688No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)