FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005093100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316471859
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA8834964427.917061655709492TruSeq Adapter, Index 12 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA70811402.237525959614627TruSeq Adapter, Index 12 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC28189630.8907468136053134TruSeq Adapter, Index 12 (100% over 62bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA18927740.5980860370905837TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA18728200.5917808951221789TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA17790910.5621640437862755TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA17764600.5613326902471919TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA17657280.5579415514477071TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA17307230.5468805363828574TruSeq Adapter, Index 12 (98% over 62bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA17097240.5402451912793927TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA17020800.537829810643606TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16920440.5346585966115869TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16872940.533157673270406TruSeq Adapter, Index 12 (98% over 62bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15296310.4833387097460694TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15219590.4809144815621664TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA8068670.2549569502165436TruSeq Adapter, Index 12 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAT3529590.1115293477010226TruSeq Adapter, Index 12 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3261860.10306951178240464TruSeq Adapter, Index 12 (98% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)